Multi-color Genomic In Situ Hybridization Identifies Parental Chromosomes in Somatic Hybrids of Diospyros kaki and D. glandulosa
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چکیده
منابع مشابه
Formamide-Free Genomic in situ Hybridization Allows Unambiguous Discrimination of Highly Similar Parental Genomes in Diploid Hybrids and Allopolyploids.
Polyploidy and hybridization play an important role in plant diversification and speciation. The application of genomic in situ hybridization (GISH) allows the identification of parental genomes in hybrids, thus elucidating their origins and allowing for analysis of their genomic evolution. The performance of GISH depends on the similarity of the parental genomes and on the age of hybrids. Here...
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Four somatic hybrids derived from a diploid wild species Solanum etuberosum and a diploid tuber-bearing Solanum clone 463-4, together with five BC1 and three BC2 plants, were analyzed by genomic in situ hybridization (GISH). None of the four somatic hybrids had the expected chromosome constitutions, i.e., 24 chromosomes from each fusion parent. Either one chromosome from S. etuberosum or one fr...
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Interspecific crossing was artificially carried out between Chlamysfarreri (Jones & Preston, 1904) ♀ and Argopectenirradiansirradians (Lamarck, 1819) ♂, two of the dominant cultivated scallop species in China. Genomic in situ hybridization (GISH) was used to examine the chromosome constitution and variation in hybrids at early embryonic stage. The number of chromosomes in 66.38% of the metaphas...
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The genomic in situ hybridization (GISH) technique was applied to Passiflora interspecific F1 HD13-133 hybrids (Passiflora sublanceolata x Passiflora foetida) and HD15-101 (Passiflora gardineri x Passiflora gibertii), and the backcrossed hybrids (BC1) HD18-106 and HD18-113 (Passiflora sublanceolata x HD13-133). GISH was performed using genomic probes prepared with the DNA from the paternal geni...
متن کامل[Genomic in situ hybridization in intergeneric hybrids between Raphanus sativus and Brassica oleracea].
Genomic in situ hybridization (GISH) was applied to study the meiosis of F1 plants from intergeneric hybrids between radish (Raphanus sativus, 2n=18, RR) and cabbage (Brassica oleracea, 2n=18, CC). The result showed that its somatic cells had the expected chromosomes, RC, 2n=18; but the pollen mother cells (PMCs) were different. There were three main kinds of PMCs. The first one was RC (2n=18),...
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ژورنال
عنوان ژورنال: HortScience
سال: 2002
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.37.1.184